Composite

Part:BBa_M50116:Design

Designed by: Alisha Birk, Jensina Froland   Group: Stanford BIOE44 - S11   (2018-04-25)


torCp based Trimethylamine N-Oxide sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1259
    Illegal XbaI site found at 1822
    Illegal XbaI site found at 2595
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1259
    Illegal XbaI site found at 1822
    Illegal XbaI site found at 2595
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1259
    Illegal XbaI site found at 1822
    Illegal XbaI site found at 2595
    Illegal AgeI site found at 2014
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid's length is 4080 base pairs

Notably it contains the parts: - BBa_M50080: a strong RBS - BBa_M50115: the torC operon - BBa_K1429000: GFP

And parts not listed in the registry including a high copy origin (pUC ORI) and an ampicillin resistance gene. As our only unique part was the BBa_M50115 torC operon, the plasmid itself was procured from from ATUM and thus has some features from this company's specific process including Bsal cloning sites at the beginning and end of this part.


Source

The torC operon had been synthetically designed and optimized, being sourced from: Simon, G., Jourlin, C., Ansaldi, M., Pascal, M., Chippaux, M., & Mejean, V. (1995). Binding of the TorR regulator to cis-acting direct repeats activates tor operon expression. Molecular Microbiology, 17(5), 971-980. doi:10.1111/j.1365-2958.1995.mmi_17050971

References